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Dear List--

I am trying to learn how to use the spectral unmixing function on the 
Zeiss 510.  I've loaded individual spectra of the three fluorophores 
that I'm using.  I then took an image of a region in which there's a lot 
of triple labeling and loaded the stored spectra to allow linear 
unmixing.  The program displays a resulting image.  However, the 
program appears not to display structures in which there are coexisting 
labels--only the few regions in which there is single-labeling.

Am I missing something?  Or is this how the program is designed to work?

Any suggestions welcome!

Thanks--

Martin Wessendorf
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [log in to unmask]