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Hi Greg,

So I think I'm leaning towards embedding the beads in tissue.  If I
do what you suggest, I'm worried about air bubbles localizing to the
beads (kinda like a tentpole phenomena).  What if I do the cryo
sectioning, and then soak the sections in detergent to "soften" them
up?

The gene gun is also an option.  Any input on that?  Would the latex
hold up to that (versus tungsten)?  Could the beads be "injected" at
various depths?

Thanks all,

Gary
--







Gary Laevsky, Ph.D.
Light Microscopy Suite Manager
Center for Research in Biological Structure
National Center for Microscopy and Imaging Research
1000 Basic Science Building
University of California, San Diego
9500 Gilman Drive
La Jolla, California 92093-0608

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