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Shalin

Bioptechs manufactures glass culture cylinders that have been used for that purpose.
They are made of glass, reuseable, autoclavable, and don't require grease or wax to seal them.
link:  
http://www.bioptechs.com/Products/Delta_T/Options/options.html#Culture%20Cylinders

We have many sizes and can make custom geometries.  
They are an easy way to constrain specimens.
Ask, and I can send a sample.

Dan



On Jun 4, 2014, at 4:15 PM, Shalin Mehta wrote:

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To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
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Hello listers,
We are imaging zebrafish embryos with 10x and the embryos just about
fit the field of view at the development stage of interest
(4hpf-30hpf).

Are there tried and tested methods for giving the embryos room to
grow, while keeping them within the field of view?

Embryos are mounted in agarose and we can make space in agarose pad
near the tail when the embryos are older. But, identifying the growth
axis at young stages (~4hpf) is difficult.

All inputs appreciated,
Thanks

Assistant Research Scientist,
Marine Biological Laboratory,
7 MBL Street, Woods Hole MA 02543, USA

website: http://mshalin.com
(office) Lillie 110, (ph) 508-289-7374.


Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16002
www.bioptechs.com
P: (724)282-7145
F: (724)282-0745
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