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Hi Paul, when I was still in the lab we were monitoring oxygen
concentration in media using an Oxylite Probe and Monitor like that
provided by Oxford Optronix
http://www.oxford-optronix.com/pO2monitors.htm(no commercial
interest).
We had this equipment because it is more commonly used for measuring
intra-tumoral oxygen in patient but we found it effective for diffused
Oxygen in media as well. The probes are expensive but flexible enough to
thread through a small bore perfusion inlet. It worked very well but again,
it requires an upfront investment in the equipment. Perhaps there are more
cost-effective ways of doing this, we were lucky to have the equipment
handy.
Best
Farid

On Mon, Jun 18, 2012 at 4:08 PM, Paul Herzmark <[log in to unmask]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
>
> We do 2P microscopy of thick tissue explants. To deliver appropriate oxygen
> and carbon dioxide levels to the deep parts of the tissue we bubble the
> medium with 95% O2 and 5% CO2. That keeps the cells as happy as when they
> are vascularized.
>
> Now we want to do the experiments with serum added to the medium, but the
> extra protein causes the bubbling medium to foam too much.
>
> Any suggestions on how to increase the dissolved O2 and CO2 without the
> foaming?
> Also, any ideas on how to monitor the resulting O2 levels?
>
> Thanks so much.
>
>
> Paul Herzmark
>
> [log in to unmask]
> Department of Molecular and Cell Biology
> 479 Life Science Addition
> University of California, Berkeley
> Berkeley, CA  94720-3200
> (510) 643-9603
>