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I have done this in the past with cotton-tipped applicators and methanol. I dislike doing this because it is difficult to get all the dust off. You simply need to start in the center of the sensor and in a circular pattern move from the center to the edge. You will need to repeat this many times using a new applicator each time until the sensor window is clean. Use very little methanol on the cotton tip. It seems it is partly by luck when you can get it entirely dust free. I have not found canned air to be useful for this purpose.
Good luck,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
1450 E Duarte Rd
Duarte, CA 91010
626-359-8111 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of F Javier Diez Guerra
Sent: Monday, June 04, 2007 8:45 AM
To: [log in to unmask]
Subject: cleaning CCD window
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We have a Coolsnap FX mono behind a Sutter
lambda-10 emission filter wheel, that is used for epifluorescence microscopy.
From time to time I check illumination flatness,
alignments, etc. However, when I look at
transmission samples using brightfield or
phase-contrast I notice small particles of dust
located always at same position of the image.
I have examined the microscope and the dust seems
to be located on the glass in front of the CCD of
the camera. Careful cleaning with anti-static
clothe and air-stream only changes the distribution of the dust particles.
After repeating several times the same operation,
I have realized that the dust comes from small
particles released in the process of screwing the
camera into the filter wheel thread.
Has anybody experienced something similar? Any
recommendation to keep clean the CCD window?
Thanks,
F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias, Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN
phone: +34 91 4978051
Fax: +34 91 4978087
e-mail: [log in to unmask]
"EMF <COH.org>" made the following annotations.
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