We have purchased the fluoro-nangold with the idea of doing both
confocal and EM on the same prep. The problem is that the fluoro-nanogold
literature states that it is not stable at temp. generally used for
polymerizing Spurrs  or Epon/Araldite resin. You can stabilize the
nanogold by silver enhancement. If you silver enhance the gold, you cover
the fluorescein molecule that you need for fluorescence.
  It seemed like a good idea but it hasn't worked for our purpose.
 
Patty Jansma                                    Tel:602-621-6671
[log in to unmask]
Arizona Research Labs Division of Neurobiology
University of Arizona
 
 
On Wed, 27 Mar 1996, Jim Pawley wrote:
 
> Try (Nanoprobes Inc.) <[log in to unmask]>,
>
> NANOPROBES, Incorporated
> 25 East Loop Road, Suite 124,
> Stony Brook, NY 11790-3350, USA
> http://www.tiac.net/users/everlast/nano/home.html
>
> >Does anyone know where can I found secondary antibodies stained both
> >with fluorescence and gold?
> >I don't who are selling that, but I think that now there exist this
> >kind of secondary antibodies.
> >
> >
> >Susanna Castel
> >Serveis Cientifico-Tecnics, Universitat de Barcelona
> >Lluis Sole i Sabaris, 1-3 08028 BARCELONA
> >Tf. 34.3.402.13.52
> >Fax. 34.3.402.13.98
> >E-mail: [log in to unmask]
>
>                    ****************************************
> Prof. James B. Pawley,                                       Ph.  608-263-3147
> Room 1235, Engineering Research Building,                    FAX  608-265-5315
> 1500 Johnson Dr., Madison, WI, 53706          [log in to unmask]