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Dear Mike,

what about using stereological methods for counting cells of
arbitrary shape, namely the disector principle? The brain tissue
specimens could be used directly, no need for isolation of cells. If
you are interested, please contact me for details.

Lucie Kubinova
_______________________________________________________
Dr.Lucie Kubinova
Department of Biomathematics
Institute of Physiology
Videnska 1083
CZ-14220 Prague 4
Czech Republic

Phone: (4202) 4106 2314
Fax: (4202) 4106 2488
E-mail: [log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>      I have a researcher here who wants to count astrocytes in brain tissue.
> We are using Image Pro Plus and have tried segmenting out the cells, but
> because of their unusual and varied shapes, we are not getting accurate
> results. Is there some feature that we can use to isolate them and do a
> count. Can anyone out there help us. Thanks in advance.
>
> Michael Goldsworthy
> Confocal Laboratory Supervisor
> Faculty of Medicine, HSC
> Memorial University
> St. John's, NL
> A1B 3V6
> (709) 777-8470
>