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Hi everyone,
I would also recommend using a PAP pen but be careful about how much you put on if you want to use high NA immersion objective lenses. I have had occasions where people have used too much and then when they mount their sections (with aqueous mount), the mountant sits in a bubble, which causes an issue if the lens has a short working distance. Sometimes, I will advise them to remove the wax carefully (and safely!) by using xylene.
Cheers,
Jacqui
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Tamara Howard
Sent: Thursday, 26 October 2017 7:09 a.m.
To: [log in to unmask]
Subject: Re: How to keep solution on slides
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Greg -
PAP pens are great (already suggested), but in a pinch you can also use a wax pencil or vaseline/petroleum jelly on a cotton swab to draw the barrier around your sections.
Tamara
...................................................
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM
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From: Confocal Microscopy List <[log in to unmask]> on behalf of GANG NING <[log in to unmask]>
Sent: Wednesday, October 25, 2017 8:43 AM
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Subject: How to keep solution on slides
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Hi all,
This might be a very simple question for many of you. We are doing IHC with dewaxed tissue sections on slides. When we incubated the tissue sections with antibodies, esp o/n at 4C, the antibody solution (with 0.1% triton) did not stay inside the circle we drew with a diamond scribe or sharpie; it spread over the slide, sometimes the sections dried. I wonder if any of you have an easy and simple way with little effort/cost to prevent solution running off the slides. Any input is highly appreciated.
Best,
Greg
----------------------------
Gang (Greg) Ning, Ph.D.
Director, Microscopy Facility
The Huck Institutes of the Life Sciences The Pennsylvania State University
N-048 MSC
University Park, PA 16802 USA
Phone: 814-863-0994
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