Hi Adrian

Not sure if what you are after is beads of a calibrated size but if you only
want to see small fluorescent dots a cheap way is to dip a permanent ink
marker (textliner type) into water for a couple of second and mount the
solution. Mixing ink from markers of different colours might even give you
dots with different spectra. When in suspension in a glass bottom dish, this
ink is an excellent sample for TIRF.

Med vänlig hälsning / Best regards
 
Sylvie
 
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Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Adrian Smith
> Sent: 14 November 2008 06:44
> To: [log in to unmask]
> Subject: Re: Beads (or other) for Confocal QC
> 
> On 14/11/2008, at 3:49 PM, Adrian Smith wrote:
> 
> > Browsing through the Invitrogen/Molecular Probes handbook I can see
> > several bead product lines that look interesting (eg FocalCheck,
> > TetraSpeck, InSpeck, PS-Speck).
> 
> Forgot to note that I was particularly interested in products that
> will work with a violet laser. The Probes products above look like
> they may not be excited well by the 405 but it is always hard to tell
> with printed spectra...
> 
> Regards,
> 
> Adrian