Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

We've tried it in plant tissues, and the biarsenical compound is lethal to
the cells at substantial dilution, and the fluorescence, if it's real, is
weak and hard to distinguish from background autofluorescence.

The postdoc who tried this has moved on to another project, so we haven't
pursued it very vigorously.  I know a couple of other groups in Australia
said they were trying it in plants, but have heard nothing, and assume it's
because they couldn't get it to work well enough to publish.

It looked great, though, in those gap junctions!

cheers,
rosemary

Dr. Rosemary White              [log in to unmask]
CSIRO Plant Industry            ph.     02-6246 5475
GPO Box 1600                        mob.  0402 835 973
Canberra, ACT 2601             fax.     02-6246 5334
Australia

> From: "Lundsten, Kelly" <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Date: Tue, 9 May 2006 18:05:35 -0400
> To: [log in to unmask]
> Subject: Re: Flash tag
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of David Knecht
> Sent: Tuesday, May 09, 2006 5:57 PM
> To: [log in to unmask]
> Subject: Flash tag
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> I wondered if anyone out there has had any experience with Tsien's
> (now INvitrogen) Flash tag system.  We made our first construct
> recently containing the tetracysteine motif, but got no fluorescence
> at all with the biarsenical compound.  We are checking expression
> now. The absence of much literature of this outside of the Tsien lab
> has me concerned that there are issues with making it work.
> Dr. David Knecht
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>