The coating is an aqueous solution of disodium metasilicate and sodium
polyphosphate, stainless steel discs (approx.70mm diameter) are soaked
in the solution and then let dry.  The thickness of the coating is
estimated to be around 1um but we are not 100% sure.  (we can use
smaller pieces of stainless steel if needed)

I have been given advice from another member that back scattered SEM
might be the way to do it.  We need to image large areas across the disc
so I thought AFM would not be that practical and I am not sure what the
Interference microscopy application mentioned is? we cannot use
transmission systems, also from Guy Cox 2nd Harmonics??  I only vaguely
remember you talking about this at the microscopy conference in NZ sorry
so I am not sure how this application works?

Liz



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Guy Cox
Sent: Monday, 23 March 2009 10:57 p.m.
To: [log in to unmask]
Subject: Re: Silcate coatings

I do think you'd need to tell us a bit more about your metasilicate
coating - its properties, expected thickness and how it is prepared.
Having said that, though, my best guess would be that second harmonic
might be a first line of attack.

                                      Guy
 


Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit,
Madsen Building F09, University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
     http://www.guycox.net <http://www.guycox.net/>  

 

________________________________

From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Elizabeth Nickless
Sent: Monday, 23 March 2009 11:10 AM
To: [log in to unmask]
Subject: Silcate coatings



Hi, 

I am investigating methods to visualise metasilicate coatings on metal.
Does anyone have any ideas on how to image the coating using confocal
microscopy?  Reflection doesn't work as the coating layer is too thin
and the metal surface uneven.  I thought possible mixing a dextran probe
in with the coating solution but wondered if anyone had any experience
or ideas for staining the coating once it had dried onto the metal
surface?


Liz 



No virus found in this incoming message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date:
22/03/2009 5:51 PM



No virus found in this outgoing message.
Checked by AVG. 
Version: 7.5.557 / Virus Database: 270.11.24/2017 - Release Date:
22/03/2009 5:51 PM
 


DISCLAIMER:
This email contains confidential information and may be legally privileged. If you are not the intended recipient or have received this email in error, please notify the sender immediately and destroy this email.
You may not use, disclose or copy this email or its attachments in any way.
Any opinions expressed in this email are those of the author and are not necessarily those of the Fonterra Co-operative Group.
http://www.fonterra.com/