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August 2012

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From:
Alberto Diaspro <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 23 Aug 2012 13:32:29 +0200
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

English version should be available...please, let me check


Il giorno 23/ago/2012, alle ore 13:17, Peng Xi <[log in to unmask]> ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Oh my dear, I will be driven crazy Alby... I cannot read Germany. But I
> will surely list it there. :)
> Peng
> 
> On Thu, Aug 23, 2012 at 6:36 PM, Alberto Diaspro <[log in to unmask]> wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> i propose confining this discussion to the far-field...what about the
>> ultramicroscope?
>> Siedentopf, H., & Zsigmondy, R. (1902). Uber Sichtbarmachung und
>> Größenbestimmung ultramikoskopischer Teilchen, mit besonderer Anwendung auf
>> Goldrubingläser. Annalen der Physik, 315(1), 1–39.
>> Il giorno 23/ago/2012, alle ore 10:17, Guy Cox <[log in to unmask]> ha
>> scritto:
>> 
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>> 
>>> Apart from Mark's suggestions, Synge proposed near-field microscopy in
>> 1928 (Philosophical Magazine 6, 356).  It was first demonstrated in visible
>> light in 1984 (D.W. Pohl, W. Denk, and M. Lanz (1984). "Optical
>> stethoscopy: Image recording with resolution λ/20". Appl. Phys. Lett. 44
>> (7): 651.) though it had been achieved in the microwave region as early as
>> 1972.  This is all way before the concept of STED had even been proposed
>> (1994) let alone demonstrated (2002).
>>> 
>>> It's not fair to say that only 'unlimited' methods are true
>> super-resolution.  Both 4-pi (proposed by Cremer & Cremer in 1971) and
>> linear structured illumination are generally regarded (and marketed) as
>> super-resolution.  Linear structured illumination was demonstrated two
>> years before STED (Gustafsson, M.G.L.  2000  Surpassing the lateral
>> resolution limit by a factor of two using structured illumination
>> microscopy. Journal of Microscopy 198 (2) , pp. 82-87).  I5 microscopy,
>> another technique offering substantial but not unlimited super-resolution,
>> was achieved even earlier (Gustafsson, Agard & Sedat, Journal of
>> Microscopy, 195, 10-16, 1999).
>>> 
>>>                                             Guy
>>> 
>>> Optical Imaging Techniques in Cell Biology
>>> by Guy Cox   2nd edition, 2012 CRC Press
>>>     http://www.guycox.com/optical.htm
>>> ______________________________________________
>>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>>> Aust. Centre for Microscopy & Microanalysis, F09,
>>> University of Sydney, NSW 2006
>>> ______________________________________________
>>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>>> Mobile 0413 281 861
>>> ______________________________________________
>>>      http://www.guycox.net
>>> 
>>> 
>>> 
>>> -----Original Message-----
>>> From: Confocal Microscopy List [mailto:[log in to unmask]]
>> On Behalf Of Peng Xi
>>> Sent: Thursday, 23 August 2012 4:59 PM
>>> To: [log in to unmask]
>>> Subject: Re: Light reading on optical nanoscopy
>>> 
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>> 
>>> Dear Mark,
>>>   I am not sure what you mean by "well before". Super-resolution is a
>>> term that usually refers to techniques that provides theoretically
>>> 'infinitely small' resolution, or down to single molecule size. That's
>> why
>>> although confocal is already better (1.4x better) in resolution, it is
>>> generally not treated as super-resolution. And so to multiphoton
>> microscopy.
>>>  If you have a better candidate on inventing super-resolution, please
>> let
>>> me and everybody know. I am sure that people are keen to know this.
>>> 
>>> 
>>> Sincerely,
>>> Peng Xi
>>> Ph. D.    Associate Professor
>>> Dept. of Biomedical Engineering, College of Engineering
>>> Peking University, Beijing, China
>>> Tel: +86 10-6276 7155
>>> Email: [log in to unmask]
>>> 
>>> 
>>> On Thu, Aug 23, 2012 at 11:51 AM, Mark Cannell
>>> <[log in to unmask]>wrote:
>>> 
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>> 
>>>> Hmm, A very myopic blog on a subject with a rich past.  It was
>> appreciated
>>>> that the Abbe 'limit' was not a limit well before Stephan Hell's work.
>>>> Suggest you might like to research the subject matter a bit deeper?
>>>> 
>>>> Cheers
>>>> 
>>>> PS I hope others don't start advertising their  'blogs' on this list, we
>>>> ban commercial 'blogs', perhaps this should be extend?
>>>> 
>>>> 
>>>> On 23/08/2012, at 1:32 AM, Peng Xi <[log in to unmask]> wrote:
>>>> 
>>>>> *****
>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>> *****
>>>>> 
>>>>> Dear List,
>>>>>   I am blogging on optical nanoscopy, in a very casual mode.
>>>>> 
>>>> 
>> http://xipeng.wordpress.com/2012/08/16/how-optical-super-resolution-is-achieved-1/
>>>>>   It is originally written in Chinese, after I gave a related plenary
>>>>> talk in May 2012. Last talk, in the noon time. And the audiences were
>>>> from
>>>>> all sorts of disciplines, from fresh graduate students to renowned
>>>>> professors. Therefore, I decided to make the talk interesting, and easy
>>>> to
>>>>> follow by everyone. It turns out to be very successful -- much better
>>>> than
>>>>> equations and diagrams. So, I decide to broadcast it by blogging. :)
>>>>>   Hope you like it.
>>>>> 
>>>>> Sincerely,
>>>>> Peng Xi
>>>>> Ph. D.    Associate Professor
>>>>> Dept. of Biomedical Engineering, College of Engineering
>>>>> Peking University, Beijing, China
>>>>> Tel: +86 10-6276 7155
>>>>> Email: [log in to unmask]
>>>> 
>>>> Mark  B. Cannell Ph.D. FRSNZ
>>>> Professor of Cardiac Cell Biology
>>>> School of Physiology&  Pharmacology
>>>> Medical Sciences Building
>>>> University of Bristol
>>>> Bristol
>>>> BS8 1TD UK
>>>> 
>>>> [log in to unmask]
>>>> 
>> 

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