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Hello Mahmood,
Paraffin crystals have strong birefringence. We used a polarizing module to visualize a paraffin embedded H&E stained tissues. https://www.nature.com/articles/s41598-021-85667-8
Best,
Michael
---
Michael Shribak
Senior Scientist
Marine Biological Laboratory
7 MBL St
Woods Hole, MA 02543
office phone: (508) 289-7242
email: [log in to unmask]
On Nov 30, 2022, at 9:35 AM, Cammer, Michael <[log in to unmask]> wrote:
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We routinely use H&E stained sections for testing on our confocals. They are flat, fluorescent, and high contrast for transmission detectors. (We never tested color imaging by serial transmission images with different lasers, but it should work, but why you'd want to do it this way?)
For instance, earlier this week I was trying techniques on our Stellaris and generated this (liver). https://www.flickr.com/photos/mcammer/52532118218/in/dateposted-public/
The FRAP tests shown here were with H&E brain. http://microscopynotes.com/spindisk/miniscanner/index.html
It also photoconverts with exposure to 405 nm light; we found the lifetime changes. I'm not sure it will be useful for anything biological, but it appears repeatable. https://twitter.com/mcammer1/status/1588633670373773314?s=20&t=hNbWc3TcpLDv3czuR-Sq-Q
I had some fun a few years ago with various cross subtractions of H&E taken with different spectral ranges with widefield fluorescence, but whereas the end products looked cool, I don’t think they were any better for identifying the tumors in the tissue from the normal tissue than other methods.
Cheers-
Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 550 First Avenue, MSB 657, New York, NY 10016
Office: 646-501-0567 Cell (DO NOT TEXT): 914-309-3270 [log in to unmask]
http://nyulmc.org/micros http://microscopynotes.com/
-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Mahmood Usman
Sent: Wednesday, November 30, 2022 8:57 AM
To: [log in to unmask]
Subject: Paraffin embedded tissue on confocal microscope
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Hello.
Am presently designing a research that will require the use of microscope. But am not sure if I can visualise a paraffin embedded H & E stained tissues with confocal microscope.
Thank you in anticipation of your response.
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