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July 2021

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From:
"Stephansky, Ryan" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 23 Jul 2021 14:55:31 +0000
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Hi Gary,

At our core we have used 100% EtOH, Sparkle, as well as chloroform for solutions to clean some lenses. For large lenses, I have found that Chloroform followed by Sparkle is best, using either glueless cotton swabs and or TexSwipes to apply the solution in a spiral pattern starting from the middle of the lens. If some solution remains, I use a hand air blaster (we call a fus-fus) to facilitate evaporation followed by an assessment and repeat if necessary. You can use a kimwipe and chloroform or ethanol to clean the exterior of the lenses, although you may want to be conservative with the amount of the solution you use.

I've included a list of materials that I use for performing microscope maintenance. Within the document I highlighted a few of the items that I mentioned above.

Happy to chat more and make any clarifications if necessary.

Ryan

Ryan Stephansky
Microscopy Resources on North Quad (MicRoN) Microscopy Imaging Specialist
77 Avenue Louis Pasteur, NRB 1030

-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Gary Laevsky
Sent: Thursday, July 22, 2021 4:25 PM
To: [log in to unmask]
Subject: Microscope cleaning

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Hi All,

We have 15 Leica S8APO stereo dissecting microscopes in our teaching lab, all of which need a good optics cleaning and cleaning of their optics carriers.

What say the masses?

Thanks 

Best,

Gary

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