CONFOCALMICROSCOPY Archives

August 1992

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Jim Barrett <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 10 Aug 1992 10:55:59 EDT
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>Perhaps you should describe your definition of "interpolating in z". I
>don't see any way of gaining resolution in the z-axis without either
>playing gain games or fully correcting the image for the PSF ala
>Sedat/Agard. To remove the elongation, I could "smear" the x-y resolution
>2-fold, thus reducing the x-y resolution. I can't sample z step sizes
>smaller than 100 nm on the MRC-600 which is what I am already using.
 
Our z-step is about 180 nanometers, the pixel size or  x/y resolution
is around 30 nanometers. In order to get a correct aspect ratio
I thought I should multiply (via trilinear interpolation) the z
slices by 6 (= 180/30).
 
-Jim

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