DAB, Diaminobenzidine histological staining shows no fluorescence at visible
wavelengths, but does show some fluorescence at UV wavelengths (particularly
if the non-precipitated DAB is not washed out properly).

Alan Hibbs

BIOCON
7 Walhalla Drive         Phone: 61 3 9876 9822    FAX: 61 3 8660 2290
Ringwood East VIC 3135        Director: Dr. Alan R. Hibbs
Australia

-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]]On Behalf Of Prins, F.A.
(PATH)
Sent: Wednesday, 25 July 2001 11:19 AM
To: [log in to unmask]
Subject: Re: Histological Dyes and fluorescence


-----Original Message-----
From: Neal Gliksman [mailto:[log in to unmask]]
Sent: dinsdag 24 juli 2001 18:56
To: [log in to unmask]
Subject: Histological Dyes and fluorescence


> Most transmitted light histological dyes seem to have a lot of
> fluorescence to them.  Could someone suggest a stain/dye that works well
> in transmitted light, but adds little fluorescence background to the
> sample.
>
> -Neal
> ***********************************************************************
> Neal R. Gliksman  Ph.D.
> Senior Application Scientist
> Universal Imaging Corporation
> One Ridgewood Place, 402 Boot Road
> Downingtown, PA 19335 USA
> www.universal-imaging.com
> ***********************************************************************
>

Neal,
try toluidine blue 01%

good luck

Frans Prins