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April 2008

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Subject:
Re: fluorescein diacetate (FDA)
From:
Randy Learish <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 9 Apr 2008 14:19:35 -0400
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On this topic, I was wondering how well the chloro-methylated dyes are 
retained.  The Probes handbook says these dyes have "mild" thiol-reactivity 
which would explain why the Tracker dyes are better retained in living cells.  
But does that mean they actually cross-link to proteins?  If I lysed the cells 
after labeling, would I see fluorescently labeled proteins on SDS-PAGE?

Randy

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