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Hi Adam,
In my experience, mechanical stability is another issue that you need to
take into account. How are you planning to mount and adjust the
dichorics/mirrors? When we built ours (which was years ago), we had to
contend with whatever  micro-optomechanical mounts we could get and they do
drift with time. Things could have improved and you want to look for
mounts/holders that have ultralow or zero drift.



On Mon, Jul 31, 2017 at 1:32 PM, Michael Giacomelli <[log in to unmask]> wrote:

> *****
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> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Adam,
>
> The main issue we have flatness, since even very flat ones will introduce
> geometric distortion.   Rather than use the high grade ones, we just
> calibrate it out by imaging a grid target with white light and then using
> imagej to compute the unwarp matrix.  Takes less than 100 ms per megapixel
> per core to unwarp two channels in matlab this way.  Make sure you have
> tip/tilt control somewhere for the dichroics as well, otherwise centering
> the cameras is very painful.
>
> Christopher Rowlands, who I think you met, also had a clever design at SPIE
> this year for 6 (or maybe 8?) channel detection that balanced distortion
> between channels to minimize the total error.  Not sure if you need
> something like that, but you could run your design by him.
>
> Mike
>
> On Mon, Jul 31, 2017 at 4:11 PM, Adam Glaser <[log in to unmask]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hello everyone,
> >
> > I was wondering if anyone could offer advice on building an in-house
> image
> > splitter for microscopy.
> >
> > We are hoping to build one to split a rectangular ROI vertically into 2-4
> > colors.  The design seems straightforward, similar to the schematic of
> the
> > Optosplit by Andor http://www.andor.com/scientific-cameras/multi-
> > wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/.
> >
> > Are there any non-obvious factors to consider when building?  We will be
> > using 2 identical tube lenses, the first to image an adjustable slit for
> > creating the cropped rectangular ROI, the second to re-focus onto our
> sCMOS
> > camera, and super resolution imaging grade dichroics from Semrock.
> >
> > Thanks,
> > Adam
> >
>