Dear,
We tested these conditions and the effect of vibration on different
cell cultures.
The results of these experiments were published in acta astronautica,
because it was a simulation of the launching conditions of the space
shuttle.
Baert P., Van Cleynenbreugel T., Vandesompele J., De Schynkel S.,
Vander Sloten J., Van Oostveldt P.: The potential (radio-)biological
impact of launch vibration. Acta Astronautica. 58, 456-463 (2006)
If you like to have a reprint, please send a personal mail.
Patrick Van Oostveldt
Quoting RICHARD BURRY <[log in to unmask]>:
> Do not for get mechanical damage! Media sloshing back and for will
> tear the cells off the substrate. It is best to fill the chamber
> completely (no air bubbles) with media before transport. This extra
> media also serves to keep the temperature more stable.
> Also it is best to transport the culture a day before use so the
> cells can get settled down again.
>
> Dick
>
> Richard W. Burry, Ph.D.
> Department of Neuroscience, College of Medicine
> Campus Microscopy and Imaging Facility, Director
> The Ohio State University
> Associate Editor, Journal of Histochemistry and Cytochemistry
> 277 Biomedical Research Tower
> 460 West Twelfth Avenue
> Columbus, Ohio 43210
> Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849
>
> ----- Original Message -----
> From: Michael Schell <[log in to unmask]>
> Date: Wednesday, February 4, 2009 6:01 pm
> Subject: Re: Cell culture transport (one hour drive) possible ?
> To: [log in to unmask]
>
>> I've transported hippocampal neurons about half an hour away,
>> but I never tried longer. To maximize your chances of success:
>>
>> 1. The best plan is to transport them between 3 and 7 days
>> after plating, before they become synaptically mature. If
>> you can bring them to the imaging location during that time and
>> allow them to recover in an incubator for a day or longer,
>> that's the best solution.
>>
>> 2. Add Hepes pH 7.2 to a final concentration of 10-20 mM
>> to each dish to be transported. Then, seal the culture
>> dishes with parafilm and place them stacked in a styrofoam box
>> for transport. Drive carefully.
>>
>> 3. Your neurons will survive best if you can raise the
>> MgCl concentration in the medium up to 10 mM just before
>> transport. This will greatly reduce deadly calcium
>> influx. However, this maneuver might not be compatible
>> with your experiment, since cultured hippocampal neurons require
>> conditioned medium to survive (you cannot just change out of the
>> Hi Mg medium into fresh medium once you get to the imaging
>> destination; having extra conditioned medium available at the
>> destination might circumvent this problem).
>>
>> Good luck,
>> Mike
>>
>>
>>
>>
>> Michael J. Schell, Ph.D., CIV, USUHS
>> Assist. Professor
>> Dept. of Pharmacology
>> Uniformed Services University
>> 4301 Jones Bridge Rd.
>> Bethesda, MD 20814-3220
>> tel: (301) 295-3249
>> [log in to unmask]
>> >>> Christophe Leterrier <[log in to unmask]>
>> 02/04/09 5:48 PM >>>
>> Dear imaging specialists,
>>
>> I'm wondering if tranporting cell cultures (low-density rat
>> hippocampal neurons, to be more specific) would be feasible from my
>> lab to an imaging facility that is between half an hour and an hour
>> away. I'm surprized how little information I could get on the
>> web. Do
>> such thing as a portable incubator exist ? Do any of you have a
>> low-tech / low-price advice to give ? I'd be glad to hear your
>> suggestions.
>>
>> Thanks a lot,
>>
>> Christophe Leterrier
>> Ionic channels neurobiology Lab
>> UMR641 - Marseille, France
>>
>>
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