CONFOCALMICROSCOPY Archives

June 1994

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Subject:
Flourochromes for FISH
From:
"Ricky Critcher (Genetics)" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 2 Jun 1994 17:43:46 +0001
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I use an MRC 600 with a Kr\AR laser mainly for FISH. My problem occurs
when I want to use 2 flourescently labelled probes eg FITC and Texas red
as I can no longer use propidium iodide as a counter stain to visualize
the chromosomes and end up with disembodied spots on the moniter. Does
any one no of a third flourochrome that would give me 2 coloured signals
and still allow for chromosome counterstaining(ie could I use Cy5)?
Cheers.
 
 
Ricky Critcher
Dept. of Genetics
Univ. of Cambridge
Downing St
Cambridge
England

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