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Dear all,

Some comments from a commercial source (Leica Microsystems) to Spectral Analysis, as requested by list users:
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We announce the availability of new spectral analysis software for COnfocal Microscopy in the current month (May 2002).
This software will work with all Leica TCS SP, SP2 and SP2 AOBS systems,
including the high-speed RS (Resonant scanner) versions.

The package completes the spectral scanning portfolio by

·   xy-Lambda, xy-Lambda-t, xy-Lambda-z scans.

·   Isolation of reference spectra from spectral scans.

·   A spectra database with literature spectra. The database can be edited and items added during the experiment.

The package contains the following multicolor restoration algorithms for crosstalk elimination:

·   Spectral Dye Separation:

transformation by using reference spectra known in advance or recorded calibration spectra.

·   Adaptive Dye Separation:

transformation with automatic identification of reference spectra from the image data set by statistical
data analysis. This way, changes in the spectra during recording (e.g. due to environment, pH changes)
are effectively detected and differences are reported to the user.
This allows utilizing the advantages of the unmixing technology in a live cell environment.

·   Manual Dye Separation:

Manual (matrix based) separation

·   The complete package works with full spectral recordings as well as with multi-channel image data,
it does not necessarily require spectral scans.

·   The output of the analysis tools can be analyzed additionally by the LCS – Multicolor option for co-localization studies.

In this context, a basic benefit for obtaining clean primary spectra
and image data are the new Leica TCS SP2 AOBS systems,
utilizing a programmable acousto-optic multi-line beam splitter.
This element improves collection efficiency over conventional beam splitters
by 20-30% and it has a neutral characteristics with no spectral modulation in the visible spectrum.
The combination of AOBS and optimized side window PMT´s for detection allow imaging with low
illumination intensity and minimum cell damage.

Best regards

Martin Hoppe
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Martin Hoppe, Ph.D.
VP Marketing & Sales
Leica Microsystems Heidelberg GmbH
Am Friedensplatz 3
D 68165 Mannheim / Germany

Phone +49 (0)621-7028-0
Cell phone: +49 (0) 172-623-0409
Fax: + 49 (0) 621-7028-1180
Email. [log in to unmask]