I don't know about autofluorescence but I do understand that antibodies, especially mAbs, may bind to antigen better in TBS. I believe I read this in a DAKO staining manual many years back.


------Original Message------
From: Guillermo Palchik
Sender: Confocal Microscopy List
To: [log in to unmask]
ReplyTo: Confocal Microscopy List
Sent: Aug 4, 2008 2:34 PM
Subject: TBS vs. PBS fluorescence

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Dear Confocalists,

I have been trying to locate a paper I had read a few years ago  
regarding the use of TBS rather than PBS for IHC, since it reduces the  
level of background fluorescence associated with the PBS. Does anybody  
know what paper I am referring to?
In addition, does anybody have any insight into whether this is  
actually true? I mean, if I do my IHC with PBS and then wash it off  
with dH20, isn't this getting rid of most of the PBS background  
fluorescence?

Thanks,

Gil Palchik

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