Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Probably Ibidi should make a slide along the lines of Ian's suggestion
- slides with chambers whose both sides are gas permeable coverslips.
As an extra, they could come with the ports similar to what opticell
uses for reagent introduction.

On 7/16/07, Ian Dobbie <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Shalin Mehta <[log in to unmask]> writes:
>
> > After a month or so, I will need to use upright and inverted confocals with
> > different capabilities located 1 hour bus journey apart.
> > I have been searching for culture vessel that allows live cell microscopy with
> > following features (decreasing order of preference).
> > 1. Can be used with both inverted and upright microscopes for live cell
> > imaging
> > 2. multiple chambers for multiplexing measurements
> > 3. ease of transportation.
>
> Our solution to this type of situation is to make our own sealed
> chambers. You take a standard 1mm slide and drill 1 or several holes
> in it. Glue a coverslip to one side. Now you have 1 or several round
> holes with a coverslip on one side. You grow your cells on small
> coverslips fill the holes with media and invert the cells onto the
> chamber and seal with wax. You need to have media that is CO2
> independent, but most cells seem to survive in these conditions.
>
> The resulting chambers are very stiff and robust. By filling the wells
> with media the cells are well protected from mechanical shocks.
> Optically both side of the chamber are a standard coverslip, with only
> 1mm of liquid between them so should work for most setups.
>
> To reuse you simply scrape of the wax, throw away your coverslip with
> cells on it and acid wash the chamber. The only problem is drilling
> the glass is a pain. Be very careful as glass dust is nasty stuff.
>
> Ian
>
>
>
> >
> > I tried samples from this:
> > 1. Nunc -- Lab-tek chambered slides - For fixed samples, it works nicely but
> > for live cells the thick bottom of slide won't work with objectives on
> > inverted microscope. It cannot be turned upside down to view adherent cells
> > under upright microscope. Not easy to transport.
> > 2. ibidi -- u-slide 6 - Works with inverted scope because bottom is
> > coverslip-like. Can be turned upside down (for using with upright) when wells
> > of all channels are sealed. But then condenser cannot come close enough
> > because slide is resting on wells and hence cannot get good illumination
> > required for taking reference images. Relatively easy to transport live cells.
> > 3. Nunc -- Opticell - (Yet to try) appears to work with both upright and
> > inverted scope. Comes with special transportation envelope. Its memberanes are
> > 75 micron thick (less than half the coverslip thickness), so I am concerned
> > about errors due to wrong coverslip thickness. Lacks multiple chambers and
> > requires large volume of reagents.
> >
> > Please share your suggestions on modifying one of this or using another
> > product?
> >
> > Regards
> > Thanks
> > Shalin
> >
> > --
> > My co-ordinates:
> > Shalin Mehta, Graduate student
> > Graduate Programme in Bioengineering, NUS, Singapore
> > Email: shalin {dot} mehta {at} gmail {dot} com
> > Blog: electricsbm.blogspot.com
> > Mobile: +65 90694182
>


-- 
My co-ordinates:
Shalin Mehta, Graduate student
Graduate Programme in Bioengineering, NUS, Singapore
Email: shalin {dot} mehta {at} gmail {dot} com
Blog: electricsbm.blogspot.com
Mobile: +65 90694182