*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

ISAC and CYTO University Present

The CYTO U Webinar Series Inaugural Webinar

Tuesday, January 14 at 12:00 p.m. EST

Using CellProfiler for Biological Image Analysis

Mark-Anthony Bray, Ph.D.
Imaging Platform, Broad Institute of MIT

Image-based screens increasingly require the use of automated microscopes which generates hundreds, if not thousands of images at a time. With such large image sets, automatic image analysis is more objective and quantitative and less tedious then visual inspection alone. This webinar is meant to instruct biologists in the use of CellProfiler, an open-source, freely-downloadable software package designed for analyzing such images via a user-friendly interface. Material will begin with an introduction of CellProfiler, followed by details for the construction and use of a pipeline for analyzing typical image data. We will also include some of the more important considerations in cellular image analysis, by demonstrating the software on sample images as a realistic use case. Lastly, we will cover measurement export and preparation for additional analysis using machine-learning tools.

At the completion of this webinar, the attendee will have knowledge of the following:
*Understand the basics of the CellProfiler environment;
*Recognize some of the basic considerations of cellular image analysis;
*Design a simple pipeline using the graphic user interface;
*Test their pipeline and adjust the settings for optimal performance.

Who Should Attend: This tutorial is intended for beginners to quantitative cellular image analysis. No programming skills are required. However, basic knowledge of fluorescence microscopy and digital image acquisition is expected.

Presenter:  Mark-Anthony Bray earned his B.S. in biomedical engineering from Tulane University in 1996 and his Ph.D. in biomedical engineering from Vanderbilt University in 2003, followed by postdoctoral work in cellular bioengineering at Harvard University. In 2008, he joined the Imaging Platform at the Broad Institute as a computational biologist under the direction of Anne Carpenter. Using his biology and image assay development expertise, he is now involved in the adaptation of image analysis software to specific image-based screens.

Register today at: http://cytou.peachnewmedia.com/<http://cytou.peachnewmedia.com/store/provider/provider09.php>

Upcoming CYTO U Webinar
Wednesday, February 19th, 12 pm EST
Integrating Flow Cytometry and Single-Cell Transcriptomics
Mario Roederer, Ph.D.
Vaccine Research Center, National Institutes of Health


CYTO University
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, and interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Learn more at http://cytou.org

CYTO U Access for ISAC Members
For current ISAC members, your user login and password to CYTO U are the same user login and password you have for the members-only section of the ISAC Web site. If you remember your user log in to the ISAC Web site but cannot remember your password, you can obtain your password from the ISAC Web site by clicking on the link "forgot your password?". If you cannot remember your username, you can contact [log in to unmask]<mailto:[log in to unmask]> for your login information.

About ISAC
ISAC is the leading scientific society focused on cytometry, the quantitative analysis of cells and cell systems. In addition to developing CYTO U, ISAC produces the annual CYTO conference, the leading international cytometry conference, publishes Cytometry, the journal of record for the field, and Current Protocols in Cytometry, the authoritative collection of validated cytometry protocols. Join us in our mission to advance cytometry at www.isac-net.org.



Michelle Butler, CAE
Executive Director
International Society for Advancement of Cytometry (ISAC)
9650 Rockville Pike
Bethesda, MD 20814
(301) 634-7454 (p)
(301) 634-7429 (f)
[log in to unmask]