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I am receiving many requests for instruction for making dishes, maybe it would make sense to post them. This is how we do it:
You would need:
1. Roper Whitney XX hand punch (Roper Whitney #135010001 with bench mounting base #139010001) to make holes in the bottom of plastic dishes.
2. Hand deburring tool (Small Parts Inc #DBR-HT)
3. Sylgard 184 glue (World Precision Instruments, #SYLG184) to glue glass coverslips to dishes. It cures overnight and is nontoxic to cells.
(From: D. Kline in Microinjections: Methods and applications, ed. DJ Carroll, 2009 Humana Press, pp 135-156)
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Angela V Klaus
Sent: Friday, December 09, 2011 10:30 AM
To: [log in to unmask]
Subject: Re: Dishes for live cell imaging
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I would love to have the instructions as well. Many thanks, Angela
Angela V. Klaus, PhD
Department of Biological Sciences
Seton Hall University
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From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Julia Edgar [[log in to unmask]]
Sent: Friday, December 09, 2011 10:19 AM
To: [log in to unmask]
Subject: Re: Dishes for live cell imaging
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Thank you, Mike. Would be great to have the instructions.
Best wishes
Julia
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Those are quite easy to make in the lab. We make them all the time and in large quantities. If interested, I can send the instructions.
Mike Model
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From: Confocal Microscopy List [[log in to unmask]] On Behalf Of Julia Edgar [[log in to unmask]]
Sent: Friday, December 09, 2011 3:07 AM
To: [log in to unmask]
Subject: Dishes for live cell imaging
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Dear All
I need to purchase glass-bottomed dishes (currently we use 35 mm Petri dishes with ~15 mm diameter glass bottom) for live-imaging of primary neural cells. I have had variable degrees of success so far, mainly because the primary neuronal cells do not adhere well to the dishes (which were coated with 0.01% poly-L-lysine).
I will be grateful to hear you recommendations for dishes and/or substrate.
Thank you in advance.
Julia Edgar
University of Glasgow
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