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Date: | Thu, 15 Dec 2011 16:53:51 +0100 |
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Hi,
Porcelain Staining Racks
ANAME
ref. 8542-E40
Could you please provide your protocol for cleaning coverslips with the
nitric acid and the nitric reference?
thanks,
Elena
On 15 December 2011 16:25, Daniel Murphy <[log in to unmask]>wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
>
> I am wondering if anyone has suggestions/thoughts/experience regarding a
> good coverslip holder/rack that is made out a material that will not be
> etched by nitric acid. In our lab we currently wash our coverslips very
> carefully, following a protocol that involves a nitric acid wash step, and
> a
> lot more. In order to make the entire process a bit more efficient and
> less
> tedious, I am looking into coverslip holders/racks that the coverslips
> could
> just sit in throughout the whole procedure (primarily for water washes,
> nitric acid washes, and a drying step. These racks would need to be:
> - low-contact so that the entire coverslip surface is well exposed for
> washes, etches, and drying steps
> - made of a material that is not corroded by nitric acid (but also not gold
> or platinum!)
> - high-capacity (able to hold 50-100 coverslips at least)
>
> Does anyone have experience using one that works really well? I'm guessing
> that there are adequate staining racks available for this, but it would be
> best to know which one has worked for other people.
>
> Any thoughts suggestions would be very much appreciated!
>
> Thanks!
>
> Dan
>
--
Elena Rebollo Ph.D.
Confocal Microscopy Unit IBMB-PCB
Molecular Biology Institute of Barcelona (IBMB), CSIC
c/ Baldiri Reixac,10-12
08028 Barcelona (Spain)
Tel (+34) 620938111/ (+34) 934020249
www.ibmb.csic.es<http://www.ibmb.csic.es/index.php?pg=laboratorio&idLaboratorio=27&tab=lab_home>
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