Jan,

check the information provided by Zeiss:

http://zeiss-campus.magnet.fsu.edu/articles/lightsources/metalhalide.html

Metal Halide lamps are quite comparable to HBO in terms of output spectra
an power, usually a bit more powerful in the blue range. Main advantages
to HBO are better stability (less flickering) and longer lifetime, which
makes them suitable for life cell imaging. Another option would be LED
illumination - less heat output, even longer lifetime, fast switching,
more expensive, fixed wavelengths.

Michael


> Hello,
> not a confocal question, but I hope someone may enlighten me.
>
> One of our users needs to replace the lamp housing on her Zeiss
> fluorescence
> microscope. The Zeiss rep recommends switching to a HXP lamp and housing.
> She needs to know that the HXP lamp will give equal or better excitation
> than HBO of especially Alexa 488 and Alexa 546.
> I have no experience of HXP lamps, so how does the excitation efficiency
> compare to a HBO lamp in practice? Other advantages, besides long life?
>
> Best regards,
> Jan Grawé
>
> ................................................
> Jan Grawé
> Cell Analysis Core Facility
> Rudbecklaboratoriet/C5
> Dag Hammarskjölds väg 20
> SE-75185 Uppsala
> SWEDEN
> Phone: +(0)18-4714656
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