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Hi Stephan 

I have been taking measurements from our 2-photon system, I have taken to
using a chroma test slide to take edge spread functions in the Z-axis.
Obviously this will not give me the lateral resolution but it is very simple
to set up there is lots of signal and bleaching doesn’t seem to be an issue.
I am able to quickly measure the resolution at any point in the field of
view, it will also show how well my system is aligned. 

Hope that helps. 

Stuart