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Hi Guosheng,
Further to the previous replies, we had a very similar problem with an Olympus FV1000 recently. Initially I thought the argon ion laser had died but it was only halfway through its usual lifetime so I suspected the AOTF was not working properly. It turned out to be the case and the Olympus engineers replaced it and it's all fine again. We were fortunate that they had a part available and could do the repair promptly.
Kind regards,
Jacqui
________________________________________
From: Confocal Microscopy List [[log in to unmask]] on behalf of Craig Brideau [[log in to unmask]]
Sent: 28 September 2019 07:23
To: [log in to unmask]
Subject: Re: [EXTERNAL] Zeiss LSM510 laser alignement or other (component) issue?
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Arvydas is right; check the AOM before you try any drastic alignment
changes. You could end up sending your beams through an unfavorable path in
a malfunctioning AOM and get very strange results. Also, any time you spend
aligning prior to replacing an AOM is basically wasted time, as you will
have to align again for a new AOM block.
Craig
On Fri, Sep 27, 2019 at 12:46 PM Arvydas Matiukas <[log in to unmask]>
wrote:
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> *****
>
> Hi Guosheng,
>
> In front of the fiber there is the AOM that attenuates intensity of
> combined VIS lasers. When I replaced argon laser I had to align only its
> beam to other VIS beams and into the AOM . In my case that was complicated
> because of slightly different laser head size. I don't think that normal
> vibration level could misalign, at least never happened in 15 years of our
> system. If you really see misalignment of all three VIS lasersthat is very
> unusual.
> I suggest to measure optical power directly at the all VIS laser
> output. If is not significantly lower then I also would suspect AOM problem
> (either crystal or driver). BTW , sometimes cooling fans of the laser
> module fail, and it overheats - check that as well.
>
> Best regards,
> Arvydas
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
>
>
>
> >>> Guosheng Liu <[log in to unmask]> 9/27/2019 2:22 PM >>>
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>
> Hello Listers,
>
> The argon scanning is barely detectable in our LSM510, but the laser
> itself should be fine. After opening the laser model box, I noticed a
> drastic alignment problem, not only for the Argon lines but also 543nm
> line! Wondering what has happened -- any vibration should not cause this
> much misalignment except someone touched them (!?).
>
> Tried a few twists at one of the mirrors to make the light (argon or
> 633nm) align to the fiber but still not get any signal. 405nm line is Ok
> since it is using a different mirror/fiber. I'm now wondering if other
> component/part in this pathway broke. Before requesting an on-site service,
> I wish to know if there is any similar experience in SLM510 community.
>
> Thanks,
>
> Guosheng
>
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