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Thank you, Sam,
It is working now
Mike
---
For the approach you are taking, you can address this via this location in SlideBook properties:
properties > advanced capture > ShutterBrightfieldTimelapse
If you are capturing a single channel transmitted capture SlideBook leaves the shutter open, unless that’s set to yes. Therefore, if you'd like to shutter during single channel transmitted capture, please set this to yes. Please let us know if this resolves your issue.
Best Regards,
-
Sam
-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of samuel connell
Sent: Saturday, June 29, 2019 11:51 AM
To: [log in to unmask]
Subject: Re: Time-lapse in Slidebook
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To join, leave or search the confocal microscopy listserv, go to:
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Good Morning Michael,
You are absolutely welcome to write an email to [log in to unmask] and the team will certainly give their best effort to help out with a quick software question (and true likely provide an invitation to upgrade your older software to the newest version.) What you are looking for can also be found either by searching in the manual or in the past 5 years or so, it can be found via pressing F1 and context-sensitive help opens right up.
Here's the section of the manual describing how to do what you are looking
for:
1.
Setting Capture Preferences to Open and Close Shutter during Capture
If you are performing single channel 3D capture, the default fluorescence shutter action in SlideBook is to leave the shutter open during capture. If you would like the shutter to open and close after every capture, select the Advanced button in the Capture Settings section of the Capture dialog box. Select the 2D/3D capture checkbox labeled Open and close shutter on single channel or simultaneous 3D capture in the General tab. The default action for multiple channel capture is to open and close the shutter after every capture.
Best Regards,
-
Sam
Samuel Connell
Director of Sales
Intelligent Imaging Innovations (3i)
3509 Ringsby Court
Denver, CO 80216 USA
1-720-437-6926
www.intelligent-imaging.com
On Sat, Jun 29, 2019 at 4:31 AM MODEL, MICHAEL <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Has anyone done transmission time-lapse acquisition using Slidebook?
> Our problem is that transmission shutter never closes, and the sample
> remains exposed to light for many hours. It is white light, not
> fluorescence, but still.(Unfortunately, 3i has a policy of not talking
> to customers without a service agreement, so I have to seek help from the confocal community...).
> Thank you!
>
> Mike Model
>
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