CONFOCALMICROSCOPY Archives

May 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Andreas Bruckbauer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 17 May 2009 14:55:13 -0500
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One nice thing about the new Olympus systems is that they now have a
motorized beam expender to adjust the beam diameter to the obejective. When
changing the objective this moves so that the back aperture is always
filled. Even when changing the wavelength the beam expander adjusts to the
changing laser diameter. The 25 objective has a correction collar for
spherical abberation which the comparable objectives from Zeiss and Leica do
not have. One has to keep in mind that it is corrected only for IR not for
visible light so that for normal confocal we still use the 20x. 
The IR laser control is still not implemented in the most recent Olympus
software, but they promised this for the next version. When you had a demo
on the older systems with Olympus pre-chirp, the beam alignment was manual,
whereas now you get a deep-see (Or Coherent vision) and everything goes
automatically. The illumination profile is quite stable with wavelength
change and there are two easily accessable micrometers to adjust it, whereas
on the LSM 710 the illumination profile wanders.

Best wishes

Andreas

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