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Hello everyone,
Thank you for your replies. The bulb predates my time here (over a year) and the screen indicating lamp use doesn't work, so it's impossible to know how many hours it's been used for.
I will check the things that some of you have mentioned, as well as replace the bulb if the rest seems fine (rather than retire it completely).
Best,
Mathew Duguay
--
Research Technician, LDI Cell Imaging Facility
& LDI Flow Cytometry Facility
LDI, Segal Cancer Centre – E415
Jewish General Hospital
3999 Côte-Sainte-Catherine
Montréal, Qc
H3T 1E2
Tel: 514-340-8222 ext. 22003
Web: http://www.ladydavis.ca/en/cell_imaging
Instrument Booking: https://corescheduler.ladydavis.ca
-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Jacqueline Ross
Sent: Tuesday, April 12, 2022 6:57 PM
To: [log in to unmask]
Subject: Re: Leica EL6000 External Light Source Issue
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Hi Matthew,
I often see this whitish residue when changing these types of bulbs, both with the Leica EL6000 and the various XCite lamps. I don’t ever see the residue on the actual bulb surface though. It’s usually just in the bottom of the lamp house and sometimes on the base of the lamp. I think this is quite normal.
However, as William has already asked, how many hours has the lamp done? Generally, these mercury metal halide lamps should be changed at 2000 hours. The other question I would have is whether you operate the lamp with a minimum run time. It is not an LED, so I would recommend that it has at least 30 minutes before being turned off and the same cool down. In fact, in our facility, we have a mandatory 1 hour on, 1 hour off rule and if the microscope is to be used within 2 hours or less, we don’t turn it off. I believe this helps with ensuring a good lifetime/stability for the bulb and also for safety, as it still does contain mercury. In saying this, some light sources won’t allow you to turn the lamp on again if it is still hot (within a certain time frame).
The fact that the bulb is going off might also be a problem with the power supply or perhaps the bulb is not seated properly or it’s overheating. I know it sounds basic but I would also check that all of the power cords are properly inserted.
Finally, if there was residue on the bulb surface, I would absolutely replace it. Although the bulbs are expensive, you are losing valuable time and there could be a safety issue here.
Kind regards,
Jacqui
Jacqueline Ross
Lead Technologist
Optical Microscopy & Image Analysis
Biomedical Imaging Research Unit (BIRU)
School of Medical Sciences
Faculty of Medical & Health Sciences
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From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Mathew Duguay
Sent: Wednesday, 13 April 2022 8:03 am
To: [log in to unmask]
Subject: Leica EL6000 External Light Source Issue
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Hello everyone,
I'm having an issue with a Leica EL6000 external light source. We use it for looking at fluorescence through the eyepiece of a Quorum Wave FX Spinning Disc system. Lately, when I turn it on, the lamp stays on for about 15-20 seconds, then shuts off (the lights on the switch and shutter indicator stay on, but the mercury metal halide bulb inside shuts off). If I switch it off then back on again, the lamp turns on but then shuts off after a little while.
When I open the lamp house, there are small white deposits all over the bulb, notably around the connectors, but also where the metal reflector connects to the plastic casing. I've removed most of it with a toothbrush and I have cleaned the connectors but the issue still persists.
I was wondering if any of you might know what these deposits are, and if you think they are the cause of the issue.
It could just be that it is time to replace the bulb, or perhaps an issue with the power supply, or indeed some other third thing, so I wanted to see if anyone had any ideas that I might try before moving forward.
Thank you,
Mathew Duguay
--
Research Technician, LDI Cell Imaging Facility & LDI Flow Cytometry Facility LDI, Segal Cancer Centre - E415 Jewish General Hospital
3999 Côte-Sainte-Catherine
Montréal, Qc
H3T 1E2
Tel: 514-340-8222 ext. 22003
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