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Dear Claire

We have an LSM510 running on the LSM software and an LSM710 running on Zen. We have no problem with the 510 but we have had a lot of communication problems with the 710 similar to what you are describing and more (eg imaging is fine then suddenly the signal becomes very weak for no apparent reason, loss of connection to the RTC...). Since we run on Vista and also had strange ghost data filling up our hard drive, we got Zeiss to reinstall Vista and Zen. It only solved the hard disk problem but not the communication problems. They have become a lot worse lately. One of the reason in our case might be the the plugs that Zeiss uses are very loose and the cables wiggle in them but I suspect this is not the only reason.

Let us know if you find anything and we will do the same.

Med vänlig hälsning / Best regards
 
Sylvie
 
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Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14183 Huddinge
Sweden
office: +46 (0) 8 5248 1107 
LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008


> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Claire Brown,
> Dr.
> Sent: 28 June 2012 16:05
> To: [log in to unmask]
> Subject: Confocal Instability
> 
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear List,
> 
> We have two Zeiss 510 confocals and two Zeiss 710 confocals all running with the
> ZEN software. We are having a lot of issues with software-hardware
> communication.
> The software will freeze up, or  it will tell you one filter is in place but you get
> interference patterns clearly showing the wrong filter is in place, sometimes the
> regions tool is not updating from one image series to the next so your ROI is not
> the correct one and so on. We are working on a local campus domain and I'm
> trying to decide if these issues are coming from something on our end or if it is
> the ZEN software.
> 
> The problems are intermittent (never happening when the Zeiss service
> representatives are here) and seem different all the time making them very difficult
> to trouble shoot.
> 
> Are other people having similar problems? Let me know as this will help us trouble
> shoot our issues.
> 
> Feel free to respond offline if you prefer.
> 
> Sincerely,
> 
> Claire
> claire dot brown at mcgill dot ca