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May 2013

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Re: Membrane Marker **vendor reply**
From:
"Kilgore, Jason" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 31 May 2013 13:50:15 -0400
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** Vendor reply **

There are two options I'd recommend:  CellMask plasma membrane stains (of which there are orange or far red options) or wheat germ agglutinin dye conjugates (of which a full range of dye options are available).  Both can be used to label live and then fix, or can label already formaldehyde-fixed cells.  

CellMask PM dyes are more uniform, but will not survive subsequent permeabilization, whereas WGA conjugates will survive (but you must label BEFORE permeabilization or you will also label internal structures).

Jason

Molecular Probes Tech Support



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Sue Penrhyn-Lowe
Sent: Friday, May 31, 2013 2:45 AM
To: [log in to unmask]
Subject: Membrane Marker

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Does anyone know of a surface membrane marker that does not internalise 
during fixation or even a surface membrane marker  that is not internalised 
when staining the membrane of fixed cells? 
Thanks Sue

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