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Hi there,

We image live bacteria with a Leica AF6500 system, fully motorized, under
the control of the LasAF 3.1.0 software. The built in autofocus algorithm
works beautifully for E. coli using a 100x objective and phase contrast
(which allows for autofocusing every position and every time point with
minimal photodamage). All you need is a good control of the mechanical and
thermal drift to be bale to set the appropriate autofocus range. I assume
that autofocus with the 40x lense would be less demanding (we really need
to go a a speciffic subcellular plane using the 100x), and therefore would
work, but it would need to be tryed out.

Cheers,

Elena

ps. IR autofocus references to the coverslip, so it may not be the best
strategy for live bacteria unless you use microfluidics...



On 7 March 2013 09:32, Pascal Weber <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Confocal not matter.
>
> I am looking for a microscope that can do focus on slides with bacteria
> without
> any intervention. I would like to use a XY plate with 8 slides, objectiv
> 40X dry.
> Does anyone have an idea? I try IR automatic focus ( Leica, Nikon, Zeiss)
> but
> anyone works correctly.  thank you
>



-- 
Elena Rebollo Ph.D.
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Advanced Fluorescence Microscopy Unit IBMB-PCB
*http://bit.ly/AfmuBcn
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Molecular Biology Institute of Barcelona (CSIC)
C/ Baldiri Reixac, 15-20, 08028 Barcelona (Spain)
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Tel (+34) 620938111/ (+34) 934020249
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