Anyone have some good suggestions for a user who is having some challenges getting complete Ab penetration into her 50 micron thick brain slices (frozen).  It appears that her Ab's are only penetrating about 15 microns or so.  I believe she is using a reasonably standard IF protocol of 4% formaldehyde and 0.1% Triton X-100.

Thanks all for the ideas,

--
Samuel A. Connell
Director of Light Microscopy
Cell & Tissue Imaging Center
St. Jude Children's Research Hospital
262 Danny Thomas Place, D1052A
Memphis, TN 38105-3678
CTIC (901) 595-3439
Office (901) 595-2536
Cell (901) 603-3162
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