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Hi,

I would like to listen opinions regarding compartimentalization of
fura-2 in cell nucleus. I know of some reports of accumulation of fluo-3
within nucleus, which was accompanied also by changes in the calcium
affinity of the dye. I have observed that nuclei of fur-loaded cells
have a stronger  fluorescence than cytosol, but the resting is slightly
lower. I guess is just a change in Kd, but I have observed that both the
rate of Ca2+ rise and the propagation of cytosolic signals within
nucleus is slower than in cytosol. If there is just a Kd increase, could
it explain that slowed kinetic?
Thanks


-- 
Dr Pedro J Camello
Dpt Physiology
Faculty of Veterinary Sciences
University of Extremadura
10071 Caceres
Spain
Ph: 927257100 Extension 1321
Fax:927257110