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You should be able to do Z stacks with the PFS if you select range rather than top/bottom

Imaging Facility Assistant
Wellcome-MRC  Stem Cell Institute
Jeffrey Cheah Biomedical Centre
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-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Csucs Gabor
Sent: 24 June 2021 12:12
To: [log in to unmask]
Subject: Re: A1R with NIS Elements

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Dear Konstantin,

I you think about it, during a z-stack the PFS needs to be deactivated, otherwise you could not do a Z-stack at all.

Greetings			Gabor



-----Original Message-----
From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Konstantín Levitskiy
Sent: 24 June 2021 13:10
To: [log in to unmask]
Subject: Re: A1R with NIS Elements

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Hi Aryeh.
Sorry but in our A1R+ when we are trying to use z-stack our PFS is automatically deactivated. That's annoying...
Best regards,
Konstantin

-----Mensaje original-----
De: Confocal Microscopy List [mailto:[log in to unmask]] En nombre de Aryeh Weiss Enviado el: jueves, 24 de junio de 2021 8:57
Para: [log in to unmask]
Asunto: A1R with NIS Elements

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We do multipoint timelapse acquisitions in multiwell plates. At each point we acquire a z-stack.
We use the PFS (perfect focus system) for each point, and it works nicely.

However, at the end of the acquisition, we get  a popup window telling us the the various time points cannot be merged because the step, range of the Z series are different. This is a minor annoyance because it means that the time points are saved in separate files. This does not happen if we do the same acquisition with the PFS disabled (but then we risk focus drift).

Is there a way to set the program to merge those files when using the PFS?

Thanks in advance
--aryeh

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: 972-3-7384051