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May 2011

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Subject:
Keeping IMF slides good for long period?
From:
Damir Sudar <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 3 May 2011 10:58:10 -0700
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Dear all,

I would really appreciate some good suggestions how to keep 
immunofluorescently labeled slides of mammalian cells good for long 
periods (3-5 months). The fluors are typically Alexa dyes or similar. Is 
freezing at -20 or -80 a good idea? Any preparation tricks that keep the 
fluorescence and the localization intact?

Thanks,
- Damir

-- 
Damir Sudar - Staff Scientist and Deputy for Technology
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [log in to unmask]
WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html

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