*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

To obtain BL2 approval from the university we had to submit a comprehensive clean-up and training protocol. We had to build a fully contained specimen stage insert that  allows the user to perfuse virus into a petri dish inside the stage insert without the risk of air-born contamination. We have to notify the other users of the core the type of pathogen that's being imaged that day.

Eric Marino
Senior Imaging Specialist
Harvard Medical School / IDI
200 Longwood Ave
WAB Room 133D
Boston, MA 02115
[log in to unmask] edu

On Jan 4, 2012, at 3:59 PM, Terri Bruce wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> That's basically our policy at Clemson. We require the group to file a copy of their containment/clean-up protocol with me for review prior to using the facility. If I have questions and concerns regarding anything, I consult our campus IBS committee. I generally accept BSL2 but nothing higher in the core.
> 
> Terri F. Bruce, Ph.D.
> Research Assistant Professor
> Manager, Jordan Hall Imaging Facility
> Department of Biological Sciences
> Clemson University
> 132 Long Hall
> Clemson, SC 29634
> Phone: (864) 656-1264, FAX: (864) 656-0435
> E-mail: [log in to unmask]
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cammer, Michael
> Sent: Wednesday, January 04, 2012 3:36 PM
> To: [log in to unmask]
> Subject: Re: pathogens in a common use confocal core facility
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> At two institutions I've been at it has been simple to accommodate BSL2 but nothing higher.  The protocols call for containment of material and have an etoh and/or bleach clean-up procedures if the containment is broken.  This works well.  
> 
> Personally, I wouldn't want potentially more dangerous material in a standard core situation.  But there may be BSL3 and BSL4 facilities that have available microscopes (a few years ago I was invited to work on a TB project in one such facility, but declined).
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: (212) 263-3208  Cell: (914) 309-3270
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Phillips, Thomas E.
> Sent: Wednesday, January 04, 2012 3:12 PM
> To: [log in to unmask]
> Subject: pathogens in a common use confocal core facility
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> I fully understand that use of pathogens requires approval of an institutional biosafety committee and that each circumstance is unique. Having said that, I am wondering if any of those of you who run a confocal core (or use pathogens in a core), have successfully implemented a biosafety plan which allows a client whose pathogens has "modest" risks could sporadically use an instrument that is widely used by other labs for no-risk, fixed samples or non-hazardous live samples. Is it crazy to try to accommodate this type of project or is it easier than it sounds? If you have experience where you got biosafety committee approval to bring a live pathogen into a core facility for imaging, I would be interested in knowing the type of agent was allowed and what you had to do to clean the room and instrument before it could be used by other clients.  Thanks and Happy New Year. Tom
> 
> 
> 
> 
> Thomas E. Phillips, Ph.D
> Professor of Biological Sciences
> Director, Molecular Cytology Core
> 2 Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> 573-882-4712 (office)
> 573-882-0123 (fax)
> [log in to unmask]<mailto:[log in to unmask]>
> 
> http://www.biology.missouri.edu/faculty/phillips.html
> http://www.biotech.missouri.edu/mcc/
> 
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
> =================================
>