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Hi Simon,

forwarding comments from our head of microinjection facility:
- because of volume, pressure, viscosity variations inside tissues/cells and
instrumental accuracy, a variation of 500% in amounts of injected material is
to be exspected (which is 'not much' considering sub-picoliter volumes, he
states).
- best method to estimate the actually delivered volume is by 
co-injection of a
radioactive marker (nasty).
- There is some publications on the issue bei Bereiter-Hahn back in the 1980s.

Cheers, jens.


Quoting "Michaud, Norman" <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal
>
> =20
>
> 	-----Original Message-----
> 	From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of simon watkins
> 	Sent: Wednesday, September 21, 2005 1:32 PM
> 	To: [log in to unmask]
> 	Subject: Injection volumes
> =09
> =09
> 	Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=3Dconfocal=20
>
> 	Hi folks, probably a dumb questions, but does anyone know how to
> calculate the volume delivered using brinkmann/eppendorf femtojet
> microinjectors?
>
> 	=20
>
> 	S
>
> 	=20
>
> 	Simon C. Watkins Ph.D. FRC Path.
>
> 	Professor, Cell Biology and Physiology and Immunology
>
> 	Director Graduate Program in Cell Biology and Physiology
>
> 	Vice Chair, Cell Biology and Physiology
>
> 	Director Center for Biologic Imaging
>
> 	BSTS 225
>
> 	University of Pittsburgh
>
> 	3500 Terrace St.
>
> 	Pittsburgh PA 15261
>
> 	Tel:412-648-3051
>
> 	Fax:412-648-2797
>
> 	URL: http://www.cbi.pitt.edu <http://www.cbi.pitt.edu/>=20
>
> 	=20
>
>



-- 
Dr. Jens Rietdorf
EMBL, Meyerhofstr.1,
D-69117 Heidelberg,
Cell Biology/Biophysics Program,
Advanced Light Microscopy Facility
Phone ++49(0)6221 387-467 FAX-306
http://www.embl.de/almf