Hello everyone,

I have recently had to restart a stage of my research due to an
accident in the lab and I was considering a change in Fluorochrome
for this new set of experiments.

I am studying the pathways between thalamus and cortex and hoping,
using the confocal microscope, to trace the pathways all the way
through a whole rat brain.

Previously I was using DiI as a fluorochrome - has anyone got any
suggestions for a better tracer.

Additionally, I would be interested in reading any papers concerning
pathway tracing using the confocal microscope, or confocal use in
general I would be most grateful if they could mail me a reprint.

Thanks

Stuart Hall
Centre for Research into Anaesthetic Mechanisms,
Department of Biomedical Science,
University of Sheffield,
Western Bank,
Sheffield.