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-----Original Message-----

From:  "Ray Gilbert" <[log in to unmask]>
Subj:  Re: Shipping cells on coverslips in 6-well plates
Date:  Tue Mar 6, 2007 10:49 am
Size:  1K
To:  "[log in to unmask]" <[log in to unmask]>


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Some companies (I'm not sure which now days) also provide agarose which requires calcium or similar ions to gel. You could ship them embedded in this then add EDTA or similar to liquefy the agarose again. Alternatively you may be able to stain and view in normal agarose if you want - I have done this many times for immunocytochemistry and other fluorescent stains without any problems.

Cheers
 
Ray G


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of [log in to unmask]
Sent: Tuesday, 6 March 2007 8:35 p.m.
To: [log in to unmask]
Subject: Re: Shipping cells on coverslips in 6-well plates

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Hi there,

Could the coverslips be frozen in the six well plates?  I've no idea  
what that might do to the adhesion, but it would certainly keep them  
still during transport.  A styrofoam box full of dry ice slurry would  
keep them at temperature, depending on the time shipping time.  You  
could also try some sort of low gel temperature agar to immobilise  
them.  I've never tried either of these, but I have had the  
frustration of cultured cells detaching from coverslips in transit, so  
let me know how you go with this.

Lachlan Walsh

Quoting "G. Esteban Fernandez" <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi all,
>
> A user needs to ship mammalian cells grown on coverslips in 6-well
> plates and fixed with methanol.  Any suggestions on how to do this so
> the cells don't detach during shipping?
>
> Thanks.
>
> -- 
> G. Esteban Fernandez, Ph.D.
>
> Associate Director
> Molecular Cytology Research Core Facility
> University of Missouri-Columbia
> (573) 882-4895
> http://www.biotech.missouri.edu/mcc/