CONFOCALMICROSCOPY Archives

February 1994

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Glen Macdonald <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 17 Feb 1994 13:07:05 -0800
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I haven't seen the Meridian slit scanner, but we did get the Bio-Rad slit
scanner in for an afternoon last winter.  It was a very impressive
instrument. You should check it out.  We put a 400 micron slice of diI
injected brain, in buffer - not cleared in any way- on a slide and were
able to focus most of the way through it.  Resolution was sufficient to
observe dendritic spines on Purkinje cells.  And all this in real-time!
        We put the same slice under a conventional fluorescent microscope
and the effect was like seeing a flashlight held up against a sheet.  Only
the surface neurons were visible, and they were poorly resolved glowing
circles.

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