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I thought there was a wide field option that allows single image (or
single optical slice if you like) deconvolution in huygens professionl.
Never tried it though.
Mariette

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Dr. M.P.C. Kemner-van de Corput,
____________________________________________

MGC - Dept. of Cell Biology & Genetics
Erasmus Medical Center
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Op Do, 13 maart, 2008 9:13 am, schreef Jens Rietdorf:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello Sarah,
>
> Huygens as far as I know does not do any '2D' deconvolution, you might
> want
> to use one of the filters provided by Huygens though. For these it is not
> necessary to provide a PSF.
>
> The problem you experience with the contribution of the leave structure
> might be addressable by a spectral un-mixing approach.
>
> Cheers, jens
>
>
> Sarah Kefayati wrote:
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello all:
>>
>> I am collecting in situ psfs from Arabdiopsis leaves.For me it is
>> impossible to collect PSF's in z direction  as I will also capture the
>> leaf structure too and I only collect data in XY plane.
>> I am wondering if it is possible to do 2D deconvolution by these
>> collected PSF s with Huygens software(we haven't get the soft ware yet
>> we would like to try this with the test licence of the software).
>>
>> I really appreciate any information and any idea.
>>
>> Sarah
>>
>>
>
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>