*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
I liquid cool mine using a Julabo F 25 circulating cooler set to 15°C. I do use an antifreeze (Julabo Thermal ED) diluted 1:10 in water but I could probably run it with just water.
— Glenn
Glenn Merrill-Skoloff
Division of Hemostasis and Thrombosis
Laboratory Manager
From: Yevgeniy Romin <[log in to unmask]<mailto:[log in to unmask]>>
Reply-To: Confocal Microscopy List <[log in to unmask]<mailto:[log in to unmask]>>
Date: Thursday, June 12, 2014 at 1:51 PM
To: "[log in to unmask]<mailto:[log in to unmask]>" <[log in to unmask]<mailto:[log in to unmask]>>
Subject: Re: Cooling Hamamatsu ORCA Flash 4.0
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Hi Kellie
This isn't exactly the answer to your question, but I feel you may benefit from me sharing my experiences, since we run into the problem of noise in the AFM force data as well. We also have an AFM that is coupled to a microscope. We discovered that the less things are plugged into the system while the AFM is imaging, the better results we get, since we eliminate as many potential noise sources as possible. We usually use our camera to find and focus on the are of interest, but before beginning our force measurements we turn off the cameras and unplug them, as well as turn off all the fluorescent and transmitted light sources. This is probably not optimal in your case, but that is how we dealt with this problem. It just seems to me that any sort of forced air cooling may still introduce noise in the force measurement data.
Hope this helps at least somewhat
Yevgeniy
________________________________________
From: Confocal Microscopy List [[log in to unmask]<mailto:[log in to unmask]>] on behalf of Kellie Beicker [[log in to unmask]<mailto:[log in to unmask]>]
Sent: Thursday, June 12, 2014 12:48 PM
To: [log in to unmask]<mailto:[log in to unmask]>
Subject: Cooling Hamamatsu ORCA Flash 4.0
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Hi all,
We have a Hamamatsu ORCA Flash 4.0 (C11440-22C) that is currently mounted on
to a combined microscope and AFM setup. The on-board fan creates too much
noise in both fluorescence imaging and AFM force data so we would like to
cool the camera with either water cooling or forced air cooling.
I am curious if anyone has done this and has knowledge of the nuances of
this process. For water cooling: What type of refrigerated circulation unit
was used? At what temperature was the system was operated? Did you use water
or antifreeze? etc. Any possible details that may not be specifically
stated in the manual or that you learned from cooling your camera would be
appreciated.
Additionally, the AFM support recommended forced air cooling for the
quietest data acquisition, but I have even less information on this. So if
anyone has air cooled this type of camera or similar, I would appreciate any
details.
Thank you in advance for your advice,
-Kellie
----------------------------------------
Kellie Beicker
Physics Research Assistant
Physics & Astronomy, CB 3255
The University of North Carolina at Chapel Hill
Email: [log in to unmask]<mailto:[log in to unmask]>
=====================================================================
Please note that this e-mail and any files transmitted from
Memorial Sloan-Kettering Cancer Center may be privileged, confidential,
and protected from disclosure under applicable law. If the reader of
this message is not the intended recipient, or an employee or agent
responsible for delivering this message to the intended recipient,
you are hereby notified that any reading, dissemination, distribution,
copying, or other use of this communication or any of its attachments
is strictly prohibited. If you have received this communication in
error, please notify the sender immediately by replying to this message
and deleting this message, any attachments, and all copies and backups
from your computer.
________________________________
This message is intended for the use of the person(s) to whom it may be addressed. It may contain information that is privileged, confidential, or otherwise protected from disclosure under applicable law. If you are not the intended recipient, any dissemination, distribution, copying, or use of this information is prohibited. If you have received this message in error, please permanently delete it and immediately notify the sender. Thank you.
|
