CONFOCALMICROSCOPY Archives

August 2023

CONFOCALMICROSCOPY@LISTS.UMN.EDU

Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
From:
Zdenek Svindrych <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 21 Aug 2023 14:24:00 +0200
Content-Type:
text/plain
Parts/Attachments:
text/plain (84 lines)
*****
To join or leave the confocal microscopy listserv or to change your email address, go to:
https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Marco,

Cheap, but not exactly straightforward is using a webcam.

First, the webcam. Nothing super fancy is needed, but the lens needs to be
something more than just a tiny pinhole. The camera pupil should be at
least 2 mm or so, so you can match it with the eyepiece exit pupil. These
webcams typically have focusing capability, as the bigger aperture means
shallower depth of focus. It's great if the webcam software allows some
control, like exposure and focus. E.g. Logitech C615 can be found on eBay
for less than $20.

Second, more tricky, you need to align the eyepiece pupil and the webcam
lens pupil with sub-millimeter precision. That's where you need to get
crafty with lots of hot glue or some 3D printing. It's great if you have a
spare eyepiece to which you can glue the webcam permanently.

Somewhat better (and more expensive) solution might be a C-mount (or
CS-mount) machine vision camera plus an eyepiece-to-Cmount adapter. Here
you need to consider the magnification of the adapter so it matches well
the sensor size of the camera. E.g. for a "1/3 inch" sensor (6 mm diagonal)
you need 0.33x adapter magnification to get the best of the 18 mm FN (field
number) of the microscope. This should be mechanically more sturdy, and you
have better control of the camera in terms of exposure time, gain, etc.

Best, zdenek




On Fri, Aug 18, 2023 at 9:15 AM Marco Tjakra <[log in to unmask]>
wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Hope you are all doing well and had a nice summer.
> We are planning to set up a dissolution experiment in our light microscope
> (leitz laborlux s leica).
> The idea (and the light microscope) is really simple, we just need to find
> an eyepiece/ocular lens that also functions as video camera (with software)
> that can record the process.
> Any recommendations please? Economic option would be much appreciated 😊
> Thank you for your help!
>
> Sincerely,
> Marco
>
>
>
>
>
>
>
>
> När du har kontakt med oss på Uppsala universitet med e-post så innebär
> det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör
> det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/
>
> E-mailing Uppsala University means that we will process your personal
> data. For more information on how this is performed, please read here:
> http://www.uu.se/en/about-uu/data-protection-policy
>


-- 
-- 
Zdenek Svindrych, Ph.D.
Research Scientist - Microscopy Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

ATOM RSS1 RSS2