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Leoncio,

Similar to Timothy's suggestion, here is a paper on optical clearing in
insects for 3D imaging (Drosophila melanogaster in this case) from McGurk
et al.:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0000834

Regards,
Graham

--
Dr Graham Wright
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

O:   +65 6407 0167
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On 16 January 2013 02:20, Craig Brideau <[log in to unmask]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I've bugged Zeiss a bit to demo their 'Scale Optimized' lens, but they
> don't have many to loan out, surprisingly. @:-)
>
> Craig
>
>
> On Tue, Jan 15, 2013 at 8:45 AM, Tim Feinstein <[log in to unmask]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Perhaps this new "miracle" reagent called ScaleA2 (
> >
http://www.riken.jp/engn/r-world/info/release/press/2011/110830_3/index.html
)
> > could help a little with imaging through the cuticle. Never tried it and
> > can't make promises, but it may be worth a try.
> >
> > cheers,
> >
> >
> > TF
> >
> > Timothy Feinstein, PhD
> > Visiting Research Associate
> > Laboratory for GPCR Biology
> > Dept. of Pharmacology & Chemical Biology
> > University of Pittsburgh, School of Medicine
> > BST W1301, 200 Lothrop St.
> > Pittsburgh, PA  15261
> >
> > On Jan 15, 2013, at 8:54 AM, Vergara, Leoncio A. wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Does anybody know of a technique to image through the cuticle of
> > mosquitoes? We were wondering if it is possible to do multiphoton
imaging
> > in whole mosquitoes (dead or alive). Any help would be greatly
appreciated.
> > Or alternative techniques like some way of partially removing the
cuticle.
> > We are intrested on the organs that participate in viral cycles (midgut,
> > malpigian tubes, ovaries, salivary glands).
> > >
> > > Leoncio
> >