Hi Arvydas,

Have you recently adjusting any components within the microscope? Laser feedback into the laser cavity can cause this as well.

Ann Haberman

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>
>Our Coherent Chameleon laser which was ocassionally
>used for 2p or 2p-DAPI excitation on LSM510 system
>recently began performing unstably, sometimes even
>not getting "modelocked".
> 
>The laser is 4 years old but was used only 1000 hrs.
>However, Coherent engineer states that lasing crystals
>may have degraded and suggest to replace them for $15-30k
>with one more year warranty.
> 
>I would appreciate any advice/comment on cheaper options
>and laser lifetime.
> 
>Thanks,
>Arvydas
>*************
> 
> 
> 
> 
>Arvydas Matiukas, Ph.D.
>Director of Confocal&Two-Photon Imaging Core Facility
>Department of Pharmacology
>SUNY Upstate Medical University
>766 Irving Ave., WH 3159
>Syracuse, NY 13210
>tel.: 315-464-7997
>fax: 315-464-8014
>email: [log in to unmask]


-- 

Ann Haberman, PhD
Department of Laboratory Medicine
Yale University School of Medicine
1 Gilbert  St.
TAC S541
New Haven, CT 06510

203-785-7349
203-785-5415 (fax)
[log in to unmask]