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Olá all,

Besides the photoconversion of DAPI, did someone have a clue of what 
could make DAPi to stain nuclei in pink?
This effect appears mainly in samples of tissues.

Good point Chloë :-)

Best,
Paula


On 28/08/2022 01:48, Chloe van Oostende wrote:
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>
> Hi all,
> Very interested topic, that got my attention over the past 5 years, with
> some Professors perplex around me. … so I’m very excited to read all these
> comments.
> Like the paper in 2013, I have found that the photo conversion will happen
> only with our x-cite 120Q mercury lamp, but using LED based or laser at
> 405, doesn’t trigger this photochemical reaction.
> Main lesson: In material and methods, illumination source  is important to
> report ( high five to QUAREP-LIMI effort!)
> Thanks Jason, Alison et all!
> Chloë
>
> Le ven. 29 avr. 2022 à 03:54, Steffen Dietzel<[log in to unmask]>  a écrit :
>
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>>
>> Am 28.04.2022 um 18:42 schrieb Roland Nitschke:
>>> Hi Everyone,
>>>
>>> just adding to that, there is another quite nice paper about the green
>>> shifting of DAPI and Hoechst dyes:
>>>
>> There is also a very nice paper from the Dobrucki group from 2013,
>> https://pubmed.ncbi.nlm.nih.gov/23418106/
>>
>> His presentation at a FOM a few years ago first alerted me to the issue.
>>
>> Steffen
>>
>>
>> --
>> ------------------------------------------------------------
>> Steffen Dietzel, PD Dr. rer. nat
>> Ludwig-Maximilians-Universität München
>> Biomedical Center (BMC)
>> Head of the Core Facility Bioimaging
>>
>> Großhaderner Straße 9
>> D-82152 Planegg-Martinsried
>> Germany
>>
>> http://www.bioimaging.bmc.med.uni-muenchen.de
>>

-- 

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